Chromosomes can be identified using whole chromosome painting probes specific to different regions along the length of each chromosome
Chromosomes can be identified using whole chromosome painting probes.
This involves fluorescence in situ hybridisation (Fish) with a series of probes that are specific to different regions along the length of each chromosome.
The Chromoprobe Multiprobe system range from Cytocell Technologies offers a unique approach for the simultaneous analysis of all chromosomes in various applications.
The range includes the OctoChrome system for whole chromosome painting.
This is ideal for use in chromosome identification, identifying marker chromosomes, detection of balanced translocations, and confirmation of banding analysis. The Chromoprobe Multiprobe comprises a disposable glass slide device that allows the independent hybridisation of probes to all 24 chromosomes on a single slide.
The device has an array of raised bosses to which DNA probes are reversibly bound.
In the OctoChrome format it features eight squared areas combined with multiple fluorescence labelling and whole chromosome libraries. Each square contains the painting probes for three different chromosomes in three contrasting colours, visible simultaneously with a Texas Red Triple filter, and provides an efficient alternative to the sequential painting of chromosomes.
The increase in area provided by the eight-square device provides for the otherwise problematic analysis of tissue samples with low mitotic index, mosaicism and clonality.
The OctoChrome system provides a cost-effective alternative to M-Fish and Sky techniques and has applications for mutagenesis and toxicology. The chromosome combinations on the Multiprobe can also aid identification of non-random chromosome rearrangements found in leukaemia.
