SH2 domain arrays allow the user to determine in a single experiment if the protein of interest binds to multiple SH2 domains
TranSignal SH2 domain arrays, available from Cambridge BioScience, have been added to the extensive multi-functional range of array-based products from Panomics.
This latest addition includes a total of 34 different human SH2 domains for rapidly visualising protein-protein interactions.
These are of particular importance in cell signalling studies, and TranSignal SH2 domain arrays allow the user to determine in a single experiment if the protein of interest binds to multiple SH2 domains.
Arrays are produced using the recombinant conserved binding sites of individual SH2 domain proteins fused with glutathione-S-transferase (GST).
Proteins are affinity-purified and immobilised onto a membrane in duplicate.
The protein to be studied can be expressed in bacteria and incubated with the membrane.
Chemiluminescence provides safe visualisation of protein binding.
Protein-protein interactions, central to cellular signal transduction, are often mediated by non-catalytic, conserved domains such as the SH2 domain.
SH2 domains can be found in a large number of proteins with diverse biochemical functions in various cellular locations, such as in enzymes, adaptor proteins, regulatory subunits of signalling proteins, scaffold proteins, transcription factors and oncogenic proteins.
These proteins play critical roles by acting as adaptors between receptors and downstream signalling molecules, transmitting signals within a cell, and regulating the kinase activity of specific proteins.
These products are available from Cambridge BioScience in the UK and Ireland only.
