These extremely sensitive reagents increase assay signal up to ten-fold over known chemiluminescent substrates
Flowgen has just launched a new family of Bold chemiluminescent
reagents for alkaline phosphatase (AP) detection.
sensitive reagents increase assay signal up to ten-fold over
known chemiluminescent substrates.
Chemiluminescence is typically
about two orders of magnitude more sensitive than fluorescence
and more than four orders of magnitude more sensitive than
This allows for lower detection limits in
standard assays such as Elisa or on blots.
In turn, this makes it
possible to study processes and detect analytes previously not
available by other detection technologies.
Low background levels
are also observed, generating very high signal to noise ratios.
Signal intensity is up to ten times brighter than other
These characteristics make Bold substrates
particularly amenable to high throughput screening and
miniaturisation where increased density of samples requires
decreased assay volumes.
In addition, those new to
chemiluminescence can forget any concerns about which substrate,
enhancer and blocker to use.
The new reagents come in
single-bottle, ready-to-use solutions that contain all the
ingredients necessary for detection.
Two substrate formulations
are available for solution-based detection in microplates or
Bold APS 540 has an emission maximum of 540nm, while
Bold APS 450 has an emission maximum of 450nm.
select to best product for optimum detection in their
Flowgen is the UK distributor of Bold
chemiluminescent reagents from Serologicals, plus a distributor
for a wide range of molecular biology products.