These extremely sensitive reagents increase assay signal up to ten-fold over known chemiluminescent substrates
Flowgen has just launched a new family of Bold chemiluminescent reagents for alkaline phosphatase (AP) detection.
These extremely sensitive reagents increase assay signal up to ten-fold over known chemiluminescent substrates.
Chemiluminescence is typically about two orders of magnitude more sensitive than fluorescence and more than four orders of magnitude more sensitive than chromogenic reactions.
This allows for lower detection limits in standard assays such as Elisa or on blots.
In turn, this makes it possible to study processes and detect analytes previously not available by other detection technologies.
Low background levels are also observed, generating very high signal to noise ratios. Signal intensity is up to ten times brighter than other commercial regents.
These characteristics make Bold substrates particularly amenable to high throughput screening and miniaturisation where increased density of samples requires decreased assay volumes.
In addition, those new to chemiluminescence can forget any concerns about which substrate, enhancer and blocker to use.
The new reagents come in single-bottle, ready-to-use solutions that contain all the ingredients necessary for detection.
Two substrate formulations are available for solution-based detection in microplates or cuvettes.
Bold APS 540 has an emission maximum of 540nm, while Bold APS 450 has an emission maximum of 450nm.
Users simply select to best product for optimum detection in their luminometers.
Flowgen is the UK distributor of Bold chemiluminescent reagents from Serologicals, plus a distributor for a wide range of molecular biology products.
