Fluorescence-based system provides simultaneous detection of two target proteins on the same membrane with sensitivity that is equal to or better than chemiluminescence
Li-Cor Biosciences announces what it says is the first system that accurately quantifies proteins for western blot analysis - the Odyssey infrared imaging station.
The Odyssey is the first fluorescence-based system that provides simultaneous detection of two target proteins on the same membrane with sensitivity that is equal to or better than chemiluminescence.
This new technology is uniquely equipped with two infrared channels for direct fluorescence detection, allowing for simultaneous probing of two separate targets on the same membrane.
Strong and weak bands on the same blot are accurately imaged without the uncertainty of the lengthy multiple exposures needed with chemiluminescence. Additionally, the elimination of background fluorescence results in clear sharp bands as opposed to the more diffuse results offered by other fluorescent or chemiluminescence imaging systems.
Infrared fluorescence detection also eliminates the need for darkrooms, film and expensive chemiluminescence substrates. Accurate quantification is also possible, since infrared detection with fluorescently labelled antibodies eliminates variability in quantification.
Simultaneous two-colour detection eliminates the variables in comparing gels, enabling faster, more precise measurement of protein phosphorylation.
The Odyssey's high signal-to-noise ratio produced by the near infrared dyes, gives results not possible with traditional fluorescence-based imaging systems.
The system includes the Odyssey Imager, reagents, and software for scan control and data analysis.
With its compact design, the Odyssey provides maximum access to your data, either in a stand alone configuration or connection through a network.
Further applications include southern blots, northern blots, protein gel documentation (2-D gel documentation) and microplate cell based assays.
