Molecular Probes's new range of fluorescently labelled 5-aminohexylacrylamido-dUTP (aha-dUTP) nucleotides are modified at the C-5 position of uridine with a unique hexylacrylamide linker
This serves as a spacer between the nucleotide and the dye which reduces interactions between them and results in brighter conjugates and increased hapten accessibility for secondary detection reagents.
The range is available labelled with Alexa Fluor 555, Alexa Fluor 647, biotin or fluorescein and can be used to generate labelled nucleic acid hybridisation probes for many molecular biology and molecular cytogenetics applications, including multicolour techniques.
The Alexa Fluor 555 and Alexa Fluor 647 dyes used to label these nucleotides are compatible with commonly used microarray scanners, and provide greater signal correlation (R2) values than the spectrally similar Cy3 and Cy5 dye pair, improving the resolution of two-colour microarray gene expression assays to 1.3-fold changes in expression.
Biotin- or fluorescein-labelled nucleic acid probes are easily detected with streptavidin conjugates or labelled anti-fluorescein antibodies respectively, and have been used in two-colour microarray assays, Southern and northern blots, colony and plaque hybridisations, DNA sequencing primer extension, DNA and RNA amplification and bead-based separation techniques.
