Selective culture medium exploits and enhances the ability of Pseudomonas aeruginosa to produce coloured and fluorescent water-soluble iron chelators
Oxoid has launched a new selective culture medium for the isolation and identification of Pseudomonas aeruginosa.
New Oxoid Pseudomonas Cetrimide Agar (CM579) exploits and enhances the ability of Pseudomonas aeruginosa to produce coloured and fluorescent water-soluble iron chelators, allowing the presence of the organism to be easily visualised on an agar plate.
The addition of Cetrimide inhibits the growth of other micro-organisms while magnesium chloride and potassium sulphate in the medium enhance the production of pyoverdin and pyocyanin in Pseudomonas aeruginosa.
If Pseudomonas aeruginosa is present, this results in yellow-green or yellow-brown colonies that fluoresce under UV light.
Pseudomonas Cetrmide Agar is recommended by both the United States Pharmacopoeia (USP) XXVI1 and the European Pharmacopoeia (EP) IV2 for use in microbial limit tests.
In addition, this medium is recommended in AOAC guidelines for the isolation of Pseudomonas aeruginosa from cosmetics and in the AOAC method for testing disinfectants on hard surfaces.
