Providing a library of ready-to-print oligos with the flexibility to spot oligos and detect signals using various printing and hybridisation techniques
Qiagen announces the release of version 3.0 Array-Ready Oligo Sets (Aros) for the human and mouse genomes.
The Human Aros version 3.0 contains 34,580 70mer probes, representing 24,650 genes and 37,123 gene transcripts.
The Mouse Aros version 3.0 contains 31,769 70mer probes, representing 24,878 genes and 32,829 gene transcripts.
Qiagen says it uses state-of the-art methodology and proprietary software for probe design.
The human and mouse Aros version 3.0 design is based on Ensembl, a cutting-edge, public, open-source database and genome browser. The Ensembl human 13.31 and mouse 14.30 databases, both based on the human and mouse genome sequencing projects, are the data sources for the human and mouse Aros version 3.0.
Probe design allows detection of alternative splicing variants using common, partial common, or individual transcript oligos.
The human and mouse Aros come with complete annotation provided by the Qiagen oligo microarray database (Omad) and Ensembl.
Omad offers comprehensive information about each oligo, and links to Ensembl, which allows access to further information, including gene ontology (GO), oligo chromosome coordinates, comparative genome analysis, and other functional annotation (InterPro, online Mendelian inheritance in man [OMIM], disease, and protein family).
The human and mouse Aros provide a library of ready-to-print oligos with the flexibility to spot oligos and detect signals using various printing and hybridisation techniques.
Each probe is melting-temperature normalised and includes a 5' amino linker to facilitate substrate attachment.
Each Aros is provided in 384-well plate format with 600pmol of product per well.
A control plate with 24 controls in 16 replicates is also included.
Aros sample sets, which include 96 optimised 70mers in 384-well plate format at 600pmol, are also available.
