This new set contains 10,898 70mer probes representing Xenopus transcripts, and provides a valuable tool for microarray analysis of Xenopus gene expression
Qiagen has released the first array-ready oligo set for the Xenopus tropicalis genome.
This new set contains 10,898 70mer probes representing Xenopus transcripts, and provides a valuable tool for microarray analysis of Xenopus gene expression.
Sequence and cluster data were provided by Professor Jim Smith of the Wellcome Trust/Cancer Research UK Gurdon Institute of Cancer and Developmental Biology in Cambridge, and groups at this institute are among the first users of the new set.
A spokesperson said, "We are very pleased to be the first groups to use this valuable resource in the analysis of gene expression and regulation in the Xenopus embryo.
We look forward to obtaining some exciting results".
Qiagen uses state-of the-art methodology and proprietary software for probe design.
Each probe is melting-temperature normalised and includes a 5' amino linker to facilitate substrate attachment.
Extensive annotation for the Xenopus array-ready oligo set will be provided on the Qiagen oligo microarray database (Omad).
The Xenopus array-ready oligo set is provided in a 384-well plate format with 600pmol of probe per well.
A control plate with 24 (12 positive and 12 negative) controls in 16 replicates is also included.
Sample sets, which contain 96 optimised 70mers in 384-well plate format at 600pmol per well, are also available.
