Suited for peptides, protein and oligonucleotide purification, delivering exceptional chromatographic performances, high capacities for biomolecules, high efficiency and excellent selectivity.
To simplify screening and method developments, Rohm and Haas Advanced Biosciences is now commercialising Amberchrom reversed phase chromatography media under the trademark Amberchrom Profile in ready to use column formats.
Standard columns are available in 4.6,10, and 22mm ID, and larger sizes are available.
Offered in 10, 20 and 30µm particles sizes, Amberchrom Profile columns are said to be ideally suited for peptides, protein and oligonucleotide purification.
Amberchrom Profile media deliver exceptional chromatographic performances, high capacities for biomolecules, high efficiency and excellent selectivity.
Delivering higher pressure capabilities, they can be used in HPLC up to 60bar for the 20µm resin and up to 100bar for the10µm resin.
Thanks to their high chemical resistance they can be easily cleaned in place (CIP) with most organic solvents and dilute acids and bases.
CIP cleaning reduces downtime spent packing and unpacking production columns for cleaning and extends the life of a prep column.
They can be operated over a wider pH range than silica products, including under basic conditions, says Rohm and Haas.
Amberchrom Profile columns in 30µm are recommended for development and scale-up of front end capture from fermentation or synthesis procedures, in 20µm for capture steps, and 10µm provide equal or superior performances to RPC silica in final product polishing steps.
Rohm and Haas says Amberchrom Profile columns enables a seamless scale up into large scale production.
