Transfection reagent combines high transfection efficiency and low cytotoxicity, allowing effective transfection of primary cells
HiPerFect transfection reagent from Qiagen has been developed for highly efficient transfection of eukaryotic cells with siRNA, even when low siRNA concentrations are used.
Transfection of the lowest effective siRNA concentration minimises the likelihood of off-target effects, says the company.
Using HiPerFect transfection reagent, highly efficient transfection and silencing have been observed in some cases with as little as 100pM siRNA.
HiPerFect can be used to transfect a wide range of cell types, including HeLa, HeLa S3, HEK 293, NIH/3T3, Huh-7, HepG2, MCF-7, T98G, Huvec, and primary human fibroblasts.
It combines high transfection efficiency and low cytotoxicity, allowing effective transfection of primary cells.
Transfection with HiPerFect can be carried out using the common procedure of seeding cells 24 hours before the day of transfection.
Alternatively, a fast-forward protocol allows cell seeding and transfection on the same day, and is particularly useful for high-throughput applications.
The unique combination of efficient knockdown using low siRNA concentrations and rapid transfection using the fast-forward protocol makes HiPerFect the ideal choice for high-throughput RNAi screening, says Qiagen.
As well as minimising off-target effects, using low siRNA concentrations also allows more experiments with less siRNA, saving costs.
In addition, the fast-forward protocol allows siRNA transfections to be easily automated.
