Kit includes ready-to-use reagents, highly specific purification, and easy-to-follow protocols for researchers who wish to start affinity purification of recombinant proteins
By providing everything required for fast, efficient purification of 6xHis-tagged proteins, the easy-to-use Ni-NTA Fast Start Kit from Qiagen is an ideal starting point for scientists looking to expand their research into the field of protein expression, but who are unfamiliar with protein purification procedures, says the company.
Each ready-to-use column in the Ni-NTA Fast Start Kit enables purification of up to 5mg protein from cleared E coli lysates in as little as 90 minutes.
Proteins can be purified using a bind-wash-elute method that may be familiar to researchers who have purified plasmids in the past.
E coli cells are lysed in the supplied lysis buffer and centrifuged to obtain a cleared lysate.
This lysate is applied to a Fast Start Column, where 6xHis-tagged proteins bind with high affinity and untagged proteins pass through.
After washing, purified proteins are eluted using buffers containing imidazole (native conditions) or of low pH (denaturing conditions).
The purification procedure and protein yield can be followed by SDS-Page, western blotting, and immunodetection using the supplied Penta-His antibody.
Qiagen says the combination of ready-to-use reagents, highly specific purification, and easy-to-follow protocols makes the Ni-NTA Fast Start Kit the tool of choice for researchers who wish to start affinity purification of recombinant proteins, but who do not want to spend money on specialised equipment or spend time learning complicated procedures.
