Hydrophilic interaction liquid chromatography (Hilic) is an extremely useful technique for the separation of polar and hydrophilic compounds via HPLC; SeQuant has prepared a tutorial guide
The column packing that is used for Hilic separations is very polar, and Zic-Hilic columns are among the most powerful of these stationary phases.
Hilic is commonly used instead of normal phase chromatography for very polar compounds because semi-aqueous mobile phases can be used.
The use of aqueous mobile phases allows for greater solubility of the sample in the eluent; this can lead to more sensitive separations.
A typical mobile phase for Hilic is acetonitrile/buffer and the composition can range from 50-95% acetonitrile, depending on the nature of the separation.
A broad range of volatile buffers can be employed, so that separations provided by a Hilic can be readily interfaced to a MS.
An additional advantage of Hilic chromatography is that the separation is scalable for preparative chromatography (ion-pairing reagents are not employed).
SeQuant has prepared a tutorial guide that describes the nature of this powerful separation tool.
The guide includes a background to the nature of Hilic sorbents, a discussion about how these sorbents provide separations that are rapid, sensitive, provide superb resolution and are readily scalable for preparative work.
In addition, the guide includes a series of application vignettes demonstrating the power of Zic-Hilic and Zic-pHilic separations of polar drugs, glycosylated peptides, purines and pyrimidines, nucleotides, peptides, tryptic digests, acrylamides, quaternary amines, organic acids, morphine and flavanoids.
