Thermosensitive alkaline phosphatase from Promega allows scientists to streamline and speed up cloning from restriction digestion, dephosphorylation to ligation without the need for any buffer swaps
In addition, thermosensitive alkaline phosphatase (TSAP) is irreversibly inactivated by heating at 74C for 15 minutes, therefore there is no DNA clean up required before proceeding directly to ligation.
TSAP catalyses the removal of 5' phosphate groups from DNA, thus preventing the recircularisation and religation of linearised cloning vector DNA during ligation.
It is effective on 3'; overhangs, 5' overhangs, and blunt ends.
It is also useful for preparing DNA for 5' end-labelling by removing existing phosphate groups from the 5' end.
TSAP is active in all Promega restriction enzyme buffers, eliminating any clean-up steps or buffer swaps.
