This week AnaSpec, one of the world's largest providers of custom and catalogue peptides, introduced twenty-five (25) new catalogue peptides
[Ala28]-beta-Amyloid (1-40)- Cat# 61968-05.
This is amino acids 1 to 40 fragment of the beta-Amyloid peptide; modified with the Ala28; replacing Lys28 residue.
Sequence: DAEFRHDSGYEVHHQALVFFAEDVGSNAGAIIGLMVGGVV.
Cys Containing b-Amyloid (1-40) Binding Peptide- Cat# 62428.
This peptide containing flanking cysteines was shown to bind b-Amyloid.(1-40) polimer form; but not monomeric b-Amyloid (1-40).
Such peptides are useful as carrier molecules to deliver therapeutic and diagnostic reagents to amyloid plaques.
Sequence: AECDWGKGGRWRLWPGASGKTEACGP.
Melittin; honey bee- Cat# 62366.
Melittin; a 26-residue bee venom peptide; is known to induce murine antibodies specific for its hydrophilic C-terminus of residues 20 to 26 and T cell responses specific for its hydrophobic mid-region of residue 11 to 19.
This peptide is the anti-inflammatory agent; it inhibits the lyme disease spirochete.
Sequence: GIGAVLKVLTTGLPALISWIKRKRQQ.
Mycobacterium Tuberculosis Protein Ag85A- Cat# 62425.
This is a T-cell immunodominant CD8 peptide of the Ag85A Mycobacterium tuberculosis protein; MHC class I H-2Ld-restricted epitope.
Because parenterally administered Mycobacterium bovis BCG vaccine confers only limited immune protection from pulmonary tuberculosis; intranasal administration of vector expressing AdAg85A represents an effective way to boost immune protection by parenteral BCG vaccination.
Sequence: LTSELPGWLQANRHVKPTGS.
BAD BH3 (103-123)- Cat# 62489.
This is amino acids 103 to 123 fragment of BAD BH3 protein.
BH3 domain of BAD is necessary for its ability to bind Bcl-2 and Bcl-XL and induce apoptosis.
Mutations in this domain of the protein inhibit binding of BAD to Bcl-2 and Bcl-XL resulting in the apoptosis arrest; and possible neoplasia.
Sequence: NLWAAQRYGRELRRMSDEFVD.
BAD BH3 (146-159); Paired- Cat# 62483.
This is amino acids 146 to 159 fragment of BAD BH3 protein.
This peptide belongs to the pro-apoptotic members of the Bcl-2 family.
This non-phosphorylated peptide may be used as a control for the BAD BH3 protein phosphorylated at Ser155 .
Phosphorylation at Ser155 inhibits the death-promoting activity of BAD.
Sequence: RYGRELRRMSDEFE.
Bax BH3 (58-71)- Cat# 62486.
This is amino acids 58 to 71 fragment of Bax BH3 protein.
Bax is a major proapoptotic member of the Bcl2 family which is required for apoptotic cell death.
Sequence: KLSECLKRIGDELD.
Bcl-2 BH3 (85-105)- Cat# 62494.
This is amino acids 85 to 105 fragment of Bcl-2 BH3 protein.
Cleavage of Bcl-2 loop domain by caspase-3 releases BH3 domain; which is a death-promoting region common to all members of Bcl-2 family.
Bcl-2 BH3 domain contains a core sequence LRQAGDFS where Leu1 and Asp6 are critical for the homerization.
Sequence: ALSPVPVVHLTLRQAGDFSRR.
Bcl-XL BH3 (85-98)- Cat# 62481.
This is amino acids 85 to 98 fragment of Bcl-XL BH3 protein.
The Bcl-XL is anti-apoptotic protein.
Sequence: AVKQALREAGDEFE.
Bid BH3 (85-98)- Cat# 62485.
This is amino acids 85 to 98 fragment of Bid BH3 protein.
The BH3 domain of Bid is believed to play an essential role in Bax activation.
Sequence: NIARHLAQVGDSMD.
Bik BH3 (56-69)- Cat# 62487.
This is amino acids 56 to 69 fragment of Bcl-2-interacting killer (Bik) BH3 domain.
Bik BH3 peptides bind to Bcl-XL and can restore Bax/Bid-induced permeabilization in the presence of Bcl-XL.
The BH3 domain of Bid is believed to play an essential role in Bax activation.
Sequence: ALALRLACIGDEMD.
Ghrelin (1-18)- Cat# 62358.
This peptide is amino acids 1 to 18 fragment of ghrelin.
Ghrelin is a potent agonist at the human growth hormone secretagogue receptor 1a (hGHSR1a).
The entire sequence of ghrelin is not necessary for its activity.
The segment Gly-Ser-Ser(n-octanoyl)-Phe appears to constitute the 'active core' required for agonist potency at hGHSR1a; and the 18 amino acid peptide displays some functions of the full length ghrelin peptide.
Sequence: GS-S(n-octanoyl)-FLSPEHQRVQQRKES.
VIR-165; Alpha1-Antitrypsin Modification (353-372)- Cat# 62434.
VIR-165 is a modified form of virus inhibitory peptide (VIRIP); which corresponds to residues 353 to 372 of C-proximal region of human alpha1-antitrypsin; the most abundant circulating serine protease inhibitor.
VIRIP inhibits a wide variety of human immunodeficiency virus type 1 (HIV-1) strains.
This modification enhances the antitrypsin ability to inhibit HIV-1infection.
Sequence: LEAIPCSIPPCFAFNKPFVF.
Adeno-Associated Virus Epitope 2 (AAV2)- Cat# 62522.
This peptide is capsid derived immunodominant adeno-associated virus 2 (AAV2); CD8 T cell epitope.
Liver toxicity observed in a clinical trial of AAV2 delivered systemically to patients with hemophilia was ascribed to killing of vector-transduced hepatocytes by capsid-specific T cells.
Sequence: VPQYGYLTL.
Catestatin; Chromogranin A (352-372); human- Cat# 62433.
Catestatin is amino acids 352 to 372 fragment of chromogranin A (parathyroid secretory protein 1); neuroendocrine secretory protein.
Catestatin is an inhibitor of catecholamine release.
Sequence: SSMKLSFRARAYGFRGPGPQ.
CKK-Insulin Receptor Substrate 1 (IRS-1) (979-989); mouse- Cat# 62544.
This peptide is amino acids 979 to 989 fragment of the murine insulin receptor substrate 1 (IRS-1) modified with additional residues Cys76; Lys77; Lys78 at the N-terminus.
It contains the phosphorylation site at Tyr983.
Sequence: CKKSRGDYMTMQIG.
Glutamate Receptor Endocytosis Inhibitor; control peptide (GluR23A)- Cat# 62548.
This control peptide is used as a mutant counterpart to glutamate receptor endocytosis inhibitor (GST - GluR23Y).
It was employed to investigate the effect of postsynaptic application of GST - GluR23Y during whole-cell recordings of CA1 neurons in hippocampal slices.
Unlike GST - GluR23Y; GST - GluR23A; is not a tyrosine phosphorylation substrate.
Sequence: AKEGANVAG.
Myelin oligodendrocyte glycoprotein (MOG 8-21)- Cat# 62369.
This peptide is amino acids 8 to 21 fragment of the myelin oligodendrocyte glycoprotein (MOG).
It induces relapsing experimental autoimmune encephalomyelitis (EAE) in mice associated with broadening of the autoimmune repertoire.
Subtle changes in MOG (8-21) dramatically influences disease susceptibility and T cell responses in vitro.
Sequence: PGYPIRALVGDEAE.
Erythropoietin Receptor Peptide (EpoR); pTyr429; acetylated; amide- Cat# 62346.
This is amino acids 423 to 438 fragment of the Erythropoietin Receptor Peptide (EpoR); phosphorylated at Tyr429.
It binds to the N-SH2 domain that has a function in regulation of the PTPase domain and thereby signal transduction.
It activates SHP-1 (previously called PTP1C; HCP; and SHPTP1); a protein-tyrosine phosphatase with two Src homology 2 (SH2) domains predominantly expressed in hematopoietic cells.
Sequence: Ac-DPPHLK-pY-LYLVVSDSK-NH2.
Histone 4 (1-20); PRMT7 Substrate; M1- Cat# 62498.
This GRG containing peptide is amino acids 1 to 20 fragment of histone 4.
It is a substrate for human protein-arginine methyltransferase 7 (PRMT7); a type II methyltransferase capable of producing symmetrical dimethyl arginine (sDMA) modifications in proteins.
Sequence: SGRGKGGKGLGKGGAKRHRK-NH2.
Histone 4-12; p300/CBP Substrate- Cat# 62500.
This 12 residues long fragment (amino acids 3 to14) of the histone 4; is also known as H4-12.
Histone acetyltransferase (HAT) p300/CBP catalyzes the acetylation of this N-terminal tail of free histone H4 at Lys5; 8; 12.
Lys8 is thought to be the preferred acetylation site in H4.
Determinants of interaction of p300 with histone 4 appear to be fully present on H4-12; the N-terminal region of the protein.
H4-12.
is functionally equal to the longer H4-21 peptide; despite the loss of nine amino acids.
Sequence: RGKGGKGLGKGA.
Histone 4-21; p300/CBP Substrate- Cat# 62499.
This sequence is amino acids 1 to 21 fragment of the histone 4.
Histone acetyltransferase (HAT) p300/CBP catalyses the acetylation of this N-terminal tail of free histone H4 at Lys5; 8; 12; 16.
Lys8 is thought to be the preferred acetylation site in H4.
Determinants of interaction of p300 with histone 4 appear to be fully present on H4-21; the N-terminal region of the protein.
Sequence: SGRGKGGKGLGKGGAKRHRKV.
NR-Box 2 Peptide- Cat# 62534.
This 13-amino-acid peptide belongs to the nuclear receptor (NR)-box 2 proteins.
NRs represent a class of regulatory factors that bind to signals; response elements; protein cofactors; and regulate target gene transcription.
This sequence includes the conserved LxxLL motif of NR-boxes.
Sequence: KHKILHRLLQDSS.
Bombolitin I- Cat# 62506.
Bombolitin I is a small amphiphilic peptide isolated from bumblebee venom.
At least 5 bombolitins have been characterized.
This peptide elicits histamine release from rat peritoneal mast cells.
Sequence: IKITTMLAKLGKVLAHV.
Influenza Virus Hemagglutinin (326-340)- Cat# 62423.
This peptide is amino acids 326 to 340 fragment of the influenza virus hemagglutinin (HA).
This HA326 peptide elicits strong gamma interferon (IFN-?) production in the intracellular cytokine assays.
Sequence: KQNTLKLATGMRNVP.
