Utilising AnaSpec's proprietary detection technologies, AnaSpec Fret-based MMP substrates were recently used in a double differential in vivo zymography study at the University of New Brunswick
In the study by Bryan Crawford, a mixture of QXL 570/5-Tamra-labelled Peptide I incorporating a red fluorophore, and QXL 520/5-Fam-labelled Peptide XIII incorporating a green fluorophore were used to detect differential breakdown of the two different MMP substrates in zebrafish.
Peptide I was broken down mostly in the maturing myotome boundaries, whereas Peptide XIII was broken down much more strongly in the notochord sheath.
Synthetic Fret peptide substrates provide a reliable method for monitoring enzyme activity by following substrate cleavage.
Through the innovative work of researchers such as Crawford, the applications of AnaSpec's Fret technologies continue to be expanded.
