Dionex has released an application note describing a new method for determining the amount of mannose-6-phosphate (M6P) in a glycoprotein.
Application note 202, entitled 'High-Performance Anion Exchange Chromatography with Pulsed Amperometric Detection Analysis of Mannose-6-Phosphate' describes the use of an ICS-3000 ion chromatography system, Carbopac analytical columns, and electrochemical detection to determine the amount of M6P in a glycoprotein and confirm its presence.
It is an important intermediate in glycoprotein production, and deficiencies in M6P can lead to numerous congenital disorders.
M6P-containing proteins are an active area of medical research, with the goals of understanding and hopefully treating such disorders.
The method described in this application note fully resolves mannose-1-phosphate (M1P) and M6P, and M6P from the protein acid hydrolysis products created during the release of M6P from the glycoprotein.
