JPK Instruments has introduced the next-generation atomic force microscopy (AFM) system, the Nanowizard II, for biological and soft matter applications.
The Nanowizard II family provides AFM functionality-like high-resolution imaging, force measurements, nanomanipulation and lithography, all integrated with advanced optical imaging provided by inverted optical microscopes.
Fluid cells and temperature control options are claimed to make it versatile for all applications where performance and ease of use is key.
Accessories such as the Cellhesion module for measurements of cell mechanics and adhesion and the Tip Assisted Optics module for advanced AFM-optical experiments are intended provide enhanced capabilities for Nanowizard II users.
Measuring single molecules demands the highest resolution in imaging and force modes, particularly in fluids.
Long-term experiments demand the utmost stability and zero drift so a closed-loop scanner is essential.
Biological samples require optical contrast enhancement methods such as phase contrast and DIC for correlation with regular optical imaging.
Fluorescence techniques such as TIRF, FRET, FCS, FRAP, FLIM, Ca2+ response and laser scanning microscopy also require a tip-scanning AFM.
The Nanowizard II AFM and optical microscope are said to be fully software integrated.
Optical filters and an infrared light source enable simultaneous operation.
Working in liquid demands that no sensitive parts be positioned below the sample level, so a sample scanning approach is not suitable.
All parts in contact with the sample are said to be easy to exchange, replace and rigorously clean.
The AFM is sealed and offers different fluid cells for environmental control.
Electronics, hardware and software allow user-defined experiments and integration with external equipment, such as electrical or optical devices.
Flexibility for the user was the key goal in the design of this next-generation BioAFM system.
