Roche Applied Science has now completed its Xcelligence real-time cell analyser product line with its latest model, the RTCA DP Instrument.
The Xcelligence system measures impedance-based signals both in label-free cellular analysis and in cell invasion/migration assays.
The RTCA DP (dual plate) instrument is applicable for flexible low-throughput research assays and uses up to three 16-well plates for simultaneous experiments.
In contrast, the RTCA SP and MP systems cover the demand for medium to high throughput, respectively.
The RTCA DP instrument provides real-time cellular analysis with a high level of flexibility for cell science researchers carrying out parallel short-term (hours) and long-term (days) assays using label-free, non-invasive impedance measuring.
The three 16-well plates of the RTCA DP can be operated flexibly by three different users; each 16-well E-plate can be monitored and analysed independently.
The RTCA DP instrument with 16-well E-plates can be placed in standard CO2 incubators, making it easy to achieve optimum cell culture conditions.
Applications include: compound-mediated and cell-mediated cytotoxicity; cell adhesion and cell spreading; cell proliferation and cell differentiation; receptor-mediated signalling; virus-mediated cytopathogenicity; constant quality control of cells; and cell invasion and migration assays.
Xcelligence RTCA instruments monitor cellular responses continuously without exogenous labels by measuring electrical impedance.
Micro electrodes integrated in the bottom of specially engineered tissue culture E-plates measure changes and provide precise quantitative information about the status of cells, including cell number, cell adhesion, cell viability and cell morphology.
Continuous impedance monitoring of cell responsivity allows researchers to pinpoint important experimental time points for more detailed downstream assays.
Impedance measurements represent a revolution in cellular surveillance compared to single conventional endpoint assays.
