The Xcelligence RTCA System from Roche Applied Science allows label-free, continuous monitoring of cell-phenotypic changes using electrical impedance as readout.
The interaction of cells with the electronic biosensors leads to the generation of a cell-electrode impedance response which indicates the status of the cells in terms of cell number, cell viability, cell morphology and cell-attachment quality.
Real-time, continuous measurement ensures the documentation of cell phenotypes in the form of time-dependent cell-response profiles (TCRP).
The real-time online aspect of the Xcelligence System has makes it possible to directly link phenotypic cellular events to the molecular and biochemical changes that occur after transfection with sirna.
Furthermore, the real-time data stream of the Xcelligence System provides a continuous, label-free measure of cell-culture quality control for transfection, simultaneously identifying potential off-target effects of the sirna.
