Anaspec has introduced two glutathione-related kits - the Sensolyte Glutathione Cellular assay kit and the Sensolyte GST Activity assay kit.
These kits complement the colorimetric and fluorimetric Sensolyte Total GSH assay kits.
The Sensolyte Glutathione cellular assay kit provides a sensitive fluorescent assay for determination of the glutathione levels in cell or tissue extracts.
The kit contains a non-fluorescent substrate, which selectively reacts with GSH in reaction catalysed by glutathione S-transferase (GST) to form a fluorescent glutathione adduct.
The resulting blue fluorescence can be monitored at Ex/Em = 390nm/480nm.
The Sensolyte GST Activity assay kit detects total GST activity in plasma, cell and tissue lysates.
It contains a non-fluorescent substrate for GST, which upon conjugation with reduced GSH generates bright fluorescence that can be detected at Ex/Em = 390nm/480nm.
GST belong to a family of enzymes that is involved in detoxification and toxification mechanisms.
They act via conjugation of reduced glutathione (GSH) with numerous substrates such as pharmaceuticals and environmental pollutants.
Anaspec said 1-4 GST enzymes are believed to be crucial to the body's defence against cancer-causing chemicals and other toxins.
These enzymes also carry out a range of functions in cells such as removal of reactive oxygen species and regeneration of S-thiolated proteins, both of which are consequences of oxidative stress.
