AnaSpec's fluorimetric assay kits for Cathepsin G are long-wavelength, homogeneous assays suitable for screening enzyme inhibitors and activators, or for continuous assay of Cathepsin G activity.
The SensoLyte 520 Cathepsin G Assay Kit contains a FRET-based (HiLyte Fluor 488/QXL 520) substrate and the SensoLyte Rh110 Cathepsin G Assay Kit, a fluorogenic substrate labelled with Rh110.
These two substrates have excitation/emission at the green wavelengths of 490/520nm.
The longer-wavelength spectra and higher extinction coefficient of HiLyte Fluor 488 and Rh110 provide greater sensitivity and less interference from other reaction components.
Cathepsin G is a neutrophil serine protease, stored in cytoplasmic granules and secreted into the extra- and pericellular space upon neutrophil activation.
Cathepsin G plays an important role in bacterial defence, acting in combination with reactive oxygen species to help degrade engulfed microorganisms inside phagolysosomes.
Besides this bactericidal effect, Cathepsin G regulates the inflammatory response by specifically altering the function of cytokines and chemokines.
Additionally, because of its role in host defence, Cathepsin G is of interest as a potential therapeutic target.
