Thermo Fisher Scientific has introduced the Velos Pro dual-pressure linear ion-trap system for small-molecule applications, including metabolism studies, at ASMS 2011.
The company said that with improved quantitative performance, faster scanning, Trap-Higher Energy Collision Dissociation (HCD) and enhanced robustness, the Velos Pro mass spectrometer extends the applicability of ion-trap workflows.
To extend its capabilities to include ultra-high resolution and accurate mass, the Velos Pro ion trap is also available in combination with Orbitrap technology as the Orbitrap Velos Pro and Orbitrap Elite hybrid mass spectrometers.
New detection electronics in the Velos Pro enable linear quantitation of up to six orders of magnitude for enhanced reproducibility and confidence in results.
Fast scanning up to 66,000Da/sec enables ultra-high-throughput analyses, compatible with the fastest U-HPLC systems, without compromising data quality.
Trap-HCD fragmentation offers complementary, triple-quadrupole-like fragmentation that facilitates structural elucidation, sequence assignment and quantitation of isobarically labelled peptides.
Redesigned ion optics, featuring neutral-blocking technology, reduce downtime and improve robustness in all applications.
Trap-HCD fragmentation enables a lower-cost, ion-trap technology-based workflow for quantitative proteomics applications.
Because Trap-HCD produces high ion intensities at low masses, a standalone ion trap can now be used to perform relative quantitation with isobarically labelled peptides, including applications requiring tandem mass tags (TMT).
For qualitative proteomics, Trap-HCD fragmentation provides greater sequence coverage and more confident sequence assignment and post-translational modification (PTM) identification.
Fast-scanning Trap-HCD complements other fragmentation methods, including collision-induced dissociation (CID), pulsed-Q dissociation (PQD) and electron transfer dissociation (ETD), producing more MS/MS spectra per analysis designed to increase potential protein and peptide identifications.
The Thermo Scientific Data Dependent Decision Tree algorithm for automated selection of the optimal fragmentation technique now incorporates Trap-HCD fragmentation.
For small-molecule applications such as metabolism studies, the Velos Pro ion trap offers a qualitative and quantitative workflow in a single flexible system.
Fast scanning and detection capabilities yield direct improvements in quantitative performance, and are said to provide richer, complementary MSn information for structural elucidation experiments across a range of applications.
Trap-HCD fragmentation offers a complementary fragmentation methodology for the identification of novel compounds, such as metabolites.
LTQ Velos and LTQ Orbitrap Velos systems can be upgraded to the Velos Pro systems, enabling customers to extend their initial investment to include the new ion-trap technology.
