Three of Sweden’s leading universities have chosen to integrate heat stabilisation of tissues samples into the upstream sample preparation of their analytical workflows
CREATE Health at the Division of Protein Technology at Lund University, the Department of Physiology and Pharmacology at Karolinska Institutet and the Department of Clinical and Experimental Medicine and Linkoping University have chosen to use Denator’s Stabilizor system. The research group of Prof.
Peter James at CREATE Health develop better methods for diagnosis and treatment of cancer, based on identified biomarkers and molecular signatures.
This work requires rapid and permanent preservation of the molecular components present at the time of tissue excision.
James commented on the incorporation of the Stabilizor system into their workflow: “At last we have a solution to be able to preserve phosphorylated proteins in clinical samples.” The research group of Assistant Professor Camilla Svensson at the Department of Physiology and Pharmacology at Karolinska Institutet center their efforts on the study of mechanisms that regulate pain signaling, with a particular focus on pain processing in the spinal cord and communication between neurons and glia in the central nervous system.
Their aim is to pinpoint novel targets for the development of new pain therapeutics.
Dr Robert Ihnatko, who is managing the proteomics branch in the group of Prof.
Anders Blomqvist at the Department of Clinical and Experimental Medicine at Linkoping University, will use the Stabilizor system to preserve the phosphorylation states in tissue samples in order to identify proteins and phosphoproteins in the research area of brain signaling affected by cancer and inflammation.
Olof Skold, CEO at Denator, states: “We are delighted that these three research groups have demonstrated such confidence in our system”.
“It is our firm belief that heat stabilization will bring additional value to the important research performed by these groups”.
“We look forward with anticipation to the results stemming from the use of heat stabilization.” A growing number of peer-reviewed publications, particularly from protein research laboratories, demonstrates the increased acceptance of Denator’s proprietary heat-stabilization technique as an essential step in upstream sample preparation.
Scientists use the company’s Stabilizor system to instantly and permanently stop biological changes, thereby preventing the enzymatic processes that alter protein states and cause sample degradation.
Heat-stabilized tissue samples can be analyzed downstream without risk of sample degradation or changes in protein and peptide profiles.