The range of 96-well format 3D spheroid cell proliferation/ viability assays is designed for cancer drug screening.
The 3D tool is primarily used to model tumour response in vitro, utilising a culture qualified 96-well formation plate alongside a specialised matrix to drive aggregation and/or spheroid formation of cells.
Once the spheroid formation is completed, the spheroid can be treated with pharmacological agents that evaluate tumour viability after drug treatment.
The tumour expansion can be visualised microscopically and can be quantitated via image analysis software for real-time and label-free evaluation.
Upon assay conclusion, cell viability mat be assessed by fluorescence using Resazurin.
The 3D spheroid fluorometric proliferation/viability assay offers an in vitro, standardised, three-dimensional, high content format for inducing multicellular tumour spheroid formation and quantitating cell viability within the spheroids in response to pharmacological treatment.
Additionally, the physiological gradients established for nutrients, oxygen and pH promote the creation of heterogeneous cell populations within proliferating cells on the spheroid surface and necrotic cells in the core.