Ambion says its new two-step process amino allyl cDNA labelling kit is more efficient and less expensive than direct incorporation of Cy-NTPS for glass array hybridisation
Ambion's new Amino Allyl cDNA labelling kit uses a two-step process - amino allyl cDNA synthesis combined with a secondary CyT dye coupling reaction - to generate fluorescently labelled cDNA from an RNA sample for use in glass array hybridisations. This two-step protocol results in consistent, uniformly labelled cDNA and is more efficient and less expensive than direct incorporation of Cy-NTPS.
Indirect labelling with the amino allyl cDNA labelling kit has many advantages over direct labelling: reverse transcriptase incorporates amino allyl-dUTP more efficiently than Cy-dNTP; Cy-dNTPs are much more expensive than monoreactive CY3 or CY5 dyes; and there is greater variation in the incorporation of Cy dyes relative to the incorporation of amino allyl-dUTP.
The Amino Allyl cDNA labelling kit contains reverse transcription reagents, oligo(dT) and random primers, RNA hydrolysis and neutralisation reagents, coupling buffer, and NucAwayT spin columns for sample clean-up.
Also included are reagents for the solubilisation of the dye esters, reagents for quenching, controls, and a detailed instruction manual. Monoreactive Cy dyes are not included.
