Expression vectors with antibiotic resistance genes are ideal for transient and long-term gene silencing studies
The use of selectable markers permits long-term gene silencing studies in cells that take up the siRNA expression vector. Changes in phenotype due to reduced gene expression that may not be readily apparent only a few days after transfection can be followed over several weeks by long-term selection.
For cell lines with low transfection efficiencies, short-term selection enriches for cells expressing the siRNA, making RNAi effects easier to see.
The pSilencer siRNA expression vectors with antibiotic resistance genes are ideal for transient and long-term gene silencing studies.
pSilencer vectors express siRNA within mammalian cells using either a U6 or H1 Polymerase III promoter. The selectable marker containing pSilencer vectors are supplied with (1) linearised and purified vector ready for ligation; (2) a DNA insert encoding a GFP-specific siRNA; (3) a circular, negative control pSilencer vector that expresses a scrambled control siRNA; and (4) 1X DNA annealing solution.
These new siRNA expression plasmids allow for the selection of cells that have taken up the plasmid and are expressing the resistance gene.
The pSilencer expression vectors are ideal for transient and long term gene silencing studies, are now available with a hygromycin resistance marker - vectors with puromycin and neomycin resistance markers available soon - select transfected cells to enrich the population of cells expressing your siRNA, efficiently express siRNA hairpins with either the human U6 or H1 polymerase III promoter, and eliminate the need to synthesise RNA oligonucleotides for RNAi experiments.
