Analytical Technologies says its YSI 8500 is the first monitor to offer in-situ measurement of dissolved CO2 in bioprocess applications, allowing effective control strategies
With the YSI 8500 CO2 monitor from Analytical Technologies, it is possible to measure dissolved carbon dioxide in situ in bioprocess development applications and successfully implement effective CO2 control strategies.
A recent study in Biotechnology Progress describes a method for controlling dCO2 in mammalian cell culture processes, using N2 sparging in combination with predetermined upper pCO2 process limits and online output trends from the YSI 8500.
This system was successfully used to maintain the stability of the cell culture at reactor scales of 30, 40, 340, and 2000 litres, with no significant drift or loss of accuracy due to sensor drifting, over a two month duration. Engineered to fit within a variety of bioreactors, the 8500 delivers precise, real-time data on dissolved carbon dioxide levels (dCO2).
Reliable monitoring and control of dCO2 are essential for successful large-scale operation as the accumulation of dCO2 becomes problematic at high viable cell concentrations.
High dCO2 concentrations have been shown to inhibit cell growth and product formation in mammalian cells and to alter the glycosylation pattern of recombinant proteins.
Based on YSI's patented opto-chemical technology, the 8500 is ideal for monitoring of dCO2 levels in critical fermentation and cell culture processes during bioprocess development applications.
By maintaining low and constant levels, the production rate of pharmaceuticals, proteins and antibodies can be significantly increased.
