Highly purified version of DNA polymerase has additional steps in its manufacturing process that significantly reduce contaminating DNA from the E coli host
Applied Biosystems has released a highly purified version of its AmpliTaq Gold DNA polymerase to help to eliminate bacterial DNA from the enzyme preparation and make advanced PCR techniques faster and less complex.
AmpliTaq Gold DNA polymerase LD (low DNA) has additional steps in its manufacturing process that significantly reduce contaminating DNA from the E coli host.
Each lot of the LD enzyme preparation undergoes rigorous quality control testing so that no more than ten copies of bacterial 16S ribosomal RNA gene sequences are present in a standard five-unit aliquot.
This virtual elimination of background DNA prevents amplification of unintended sequences.
When combined with GeneAmp 10X Gold buffer, AmpliTaq Gold DNA polymerase LD provides a flexible hot-start system that is optimised for all PCR applications, especially for those where the elimination of bacterial DNA is critical.
