LC-ESI-Maldi system for LC-based expression proteomics workflows enables a seamless proteomics workflow with Tof/Tof systems at high sensitivity
Bruker Daltonics announces its new Proteineer-LC, a new LC-ESI-Maldi system for LC-based expression proteomics workflows. Bruker Daltonics also introduces the Top-Down Proteineer capability on its Apex-Qe Q-q-FTMS system, allowing identification, primary structure determination and localisation of post-translation modifications (PTMs) directly at the protein level, without the need for enzymatic digests.
Proteineer-LC combines multi-LC-MS/MS proteomics approaches, eg using ESI ion trap mass spectrometry, with a new optimised sample splitting and Maldi fraction collection device, called Proteineer-FC.
This enables a seamless LC- Maldi-MS/MS proteomics workflow with Tof/Tof systems at high sensitivity, using Bruker Daltonics's Anchorchip sample plate technology. Information-dependant workflows can be utilised so that tentative protein IDs can be confirmed in an intelligent, results- driven way by Warp-LCT software.
Warp-LC can analyse LC-MS(n) results from HCTplus high capacity ion trap on the fly, and then direct targeted Maldi-Tof/Tof experiments, based on the initial database search results.
The combined information significantly increases ID success rates, allows identification of lower abundance proteins and enables further PTM characterisation without LC time constraints using the LC-Maldi-Tof/Tof workflow.
Top-Down Proteineer is now available on the Apex-Qe hybrid Q-q-FTMS system launched earlier this year.
It combines the analytical power of FTMS equipped with a hybrid ESI-Q-q interface to perform top-down analysis of intact proteins in the FTMS analyser cell, thus avoiding digestion and additional separation steps.
The enormous resolving power of the FTMS is ideal for measuring protein fragment ions generated directly with electron-capture dissociation (ECD) in the FTMS analyser cell, and can deliver high fidelity long sequence runs, as well as information on the localisation of PTMs, says the company.
