The new DicerT siRNA Construction Kit allows easy and cost-effective generation of large numbers of small interfering RNAs from full-length target genes
The new DicerT siRNA Construction Kit is the latest addition to the Gene Therapy Systems range available from Cambridge BioScience.
The Dicer kit allows easy and cost-effective generation of large numbers of small interfering RNAs (siRNAs) from full-length target genes.
Compared to conventional siRNA construction methods such as chemical synthesis and hairpin siRNA expression vectors, the Dicer siRNA Construction Kit offers several advantages: a mixture of siRNAs has a better chance of success than a single siRNA design; time and money are saved through the avoidance of failed siRNA designs; and it is possible to screen more gene regions for silencing.
The Dicer siRNA Construction Kit mimics the natural RNA interference process by using recombinant human dicer enzyme, a double-stranded RNA-specific endonuclease, to cleave in vitro transcribed double-stranded (dsRNA) templates into a pool of 22 bp siRNAs. GTS Dicer kits contain sufficient material for transcribing, cleaving, and transfecting siRNAs for 50 experiments in 24-well plates with up to 5 different genes.
siRNAs are 21- to 23-nucleotide RNA molecules that can cause targeted gene silencing in mammalian cells through a process known as RNA interference.
In nature, siRNAs are generated by ribonuclease III cleavage of longer dsRNAs.
When dsRNAs are transfected directly into mammalian cells, they activate the interferon system and provoke non-specific gene suppression and cytotoxic response. siRNAs have proven to be effective at specifically silencing gene expression without causing any interferon response.
