A new technical note from ESA Analytical describes a validated method for the sample preparation and analysis of fat soluble vitamins in serum
Using the CoulArray detection system, ESA's Vitascan methodology provides a new, highly sensitive and selective HPLC method for the simultaneous determination of fat soluble vitamins including retinol (vitamin A), retinyl palmitate, vitamin E, beta-carotene, lutein, lycopene and CoQ10.
Offered as a ESA laboratory service or a methodology to be performed on a CoulArray HPLC detection system the validated procedures followed are in accordance with CLIA 88 and NIST Micronutrient Measurement QA Programme protocols.
With its multi-channel electrochemical detection array, the CoulArray HPLC detection system has the dynamic range and detection capabilities to permit the simultaneous determination of all the fat soluble vitamins mentioned.
This provides for considerable time savings and improvements in sample throughput over using traditional wet chemical or spectroscopic HPLC detection methods.
Along with the Vitascan analysis, ESA Analytical can provide customers with technical advice to ensure the most reliable results from using this methodology.
Information is available on the most appropriate time to collect blood samples; blood sampling volumes and procedures; sample handling precautions; stability issues; storage protocols; sample transfer and shipping considerations and precautions to take during sample preparation and analysis. Further useful information including vitamin reference ranges and typical chromatograms are also freely available.
Benefiting from the ultra-high sensitivity of the CoulArray electrochemical detector the extremely low endogenous levels of Vitamin K1 can also be reliably determined.
A separate validated methodology is available for this analysis.
