Cells are classified and counted based on three individual measurements of each cell, including the fluorescence intensity values for the live and dead cell based on uptake of dyes
Guava Technologies, a biomedical products company focused on the life science R and D and clinical diagnostics markets, has launched ViaCount EasyFit.
EasyFit is an innovative cluster analysis algorithm providing more precise, accurate and automated absolute cell counting and viability measurements.
Accuracy, reproducibility, and standardisation are essential to successful outcomes in laboratories that perform cell count and viability assessments.
Traditional methods for counting cells and assessing cell viability are time-consuming.
Moreover such methods often produce variable results since they rely on inherently subjective visual discrimination of dye-stained cells using a hemacytometer and microscope.
Guava's ViaCount assay is a simple, accurate, and reproducible test for absolute cell counting and viability measurements that overcomes many of the limitations of traditional methods.
The ViaCount assay reduces statistical variation by sampling large numbers of single cells, and minimises operator variability by using fluorescence intensity measurements to categorise cells as live, dead, or apoptotic. This simple and obust Guava ViaCount assay has now been enhanced with the new Guava EasyFit software feature that automates and standardises cell counting and viability assessments. "EasyFit provides users of our product with a means to operate the system without the need for manual adjustment of gating parameters", stated Keith Olson, director of product commercialisation at Guava Technologies.
"Guava ViaCount remains one of the most popular applications used on our platforms, and the enhancements introduced by this product are a direct response to feedback from our customers".
This new Guava ViaCount software provides a powerful computer-assisted clustering algorithm to group cellular distributions based on their positions in a 3D matrix.
Cells are classified and counted based on three individual measurements of each cell, including the fluorescence intensity values for the live and dead cell based on uptake of the dyes in Guava's ViaCount solution.
