Equilibrium dialysis is recognised as the preferred technique for molecular binding studies as it allows for the direct assay of molecular interactions under normal physiological conditions
Harvard Apparatus introduces the 96-well equilibrium Dialyzer for ligand binding experiments including serum-protein binding, protein-drug binding, protein-protein binding and protein-DNA binding assays.
Equilibrium dialysis is recognised as the most preferred technique for molecular binding studies as it allows for the direct assay of molecular interactions under normal physiological conditions.
Equilibrium dialysis has also shown itself to be a valuable technique for sample preparation before candidate analysis on a mass spectrometer.
Studies have shown increased target identification when equilibrium dialysis is used prior to the spectroscopy.
Each well in the plate consists of two chambers separated by a regenerated cellulose acetate membrane with a molecular weight cut off of either 5000 or 10,000 Daltons. Each chamber holds up to 250ul of sample or buffer.
During dialysis the plate needs to be rotated through 360deg in a vertical position.
Harvard Bioscience can also supply a one- or two-plate rotator with a clamp attachment for ease of use or an eight plate heated rotator.
The 96-well equilibrium Dialyzer was designed with SBS footprint and standard well-spacing to meet automation needs and is intended for single-use.
Other equilibrium dialysis products from Harvard Apparatus include the single (double chambered) disposable equilibrium Dialyzers for volumes up to 100ul, the single re-usable equilibrium Dialyzers for samples from 25 to 500ul and the re-usable multi-equilibrium Dialyzers for up to 20 samples with volumes from 250ul to 5ml.
