Using these columns for this type of rapid GPC sample clean-up enables relatively high molecular weight compounds to be resolved from the low molecular weight compounds of interest
Polymer Laboratories (PL) has published a new technical bulletin entitled 'Single stage extract clean-up in pesticides determination'.
Removal of organic matrices from extracts prior to GC/MS analysis of pesticides and other pollutants improves chromatographic efficiency, and extends the lifetime of the GC column.
High performance gel permeation chromatography (GPC) provides a simple one stage clean-up procedure for the determination of pesticides in a variety of organic matrices, eg soil, animal tissue, etc Preparative GPC is preferred for this type of sample clean-up, as higher sample loadings and fraction yields can be obtained.
PL EnviroPrep GPC columns have a relatively small pore size (100A) and low exclusion limit (4000g/mol), and so are ideal for this type of application.
EPA method SW846-3640 describes the application of preparative GPC in the clean-up of soil extracts, the application is also in the US EPA CLP statement of work for organics analysis document number OLM01.0.
PL EnviroPrep columns offer high resolution and high loading through optimisation of the particle size distribution. Using PL EnviroPrep columns for this type of rapid GPC sample clean-up enables relatively high molecular weight compounds to be resolved from the low molecular weight compounds of interest.
