Reverse transcriptase is optimised for the efficient and robust synthesis of first-strand cDNA from RNA for further amplification
Ideal for all molecular biologists performing reverse transcription (RT) and RT-PCR, the ImProm-II reverse transcriptase is optimised for the efficient and robust synthesis of first-strand cDNA from RNA for further amplification.
The ImProm-II enzyme can be used to reverse transcribe RNA templates starting with either total RNA or poly(A) +mRNA.
A fully optimised reaction buffer coupled with a powerful reverse transcriptase enzyme makes it possible to carry out reliable full-length cDNA synthesis for the reproducible analysis of rare or long messages.
Versatile and efficient, ImProm-II can be used to reverse transcribe RNA templates of over 8.5 kilobases and can be used to incorporate standard and amino-allyl modified nucleotides, typically used in the preparation of Cy3 and Cy5 labelled fluorescent probes.
cDNA synthesis conditions have been formulated for stand-alone applications or for the easy transition to gene-specific target amplification.
Fully scaleable, volumes from 1µl to 10µl of the reverse transcription reaction can be directly amplified using Promega Taq DNA Polymerase in coupled or uncoupled PCR reactions.
