Universal tissue kits provide manual and automated, high-throughput RNA purification from all types of animal tissue, including difficult-to-lyse fibrous and fatty tissues
Qiagen RNeasy 96 universal tissue kits provide manual and automated, high-throughput RNA purification from all types of animal tissue, including difficult-to-lyse fibrous and fatty tissues.
The procedure results in high yields of total RNA, ready to use in all downstream applications, including real-time RT-PCR and array analysis.
In the RNeasy 96 universal tissue procedure, efficient high-throughput disruption using the TissueLyser system provides parallel disruption and homogenisation of up to 2x96 samples in Qiazol lysis reagent.
The combination of Qiazol and RNeasy technologies results in highly pure RNA without phenol carryover.
The manual procedure can be carried out using a combination of vacuum and spin technology or using spin technology alone.
An automated format of the RNeasy 96 universal tissue kit is available for use with the BioRobot gene expression real-time RT-PCR system.
All steps from transfer of the aqueous phase to elution of pure RNA can be performed by the workstation.
