Combining HPLC column compartment with divinylbenzene-based columns allows it to reach its true potential as a separation device
Selerity Technologies is working with Jordi Associates to provide rugged polymeric based columns for high temperature use.
By using stationary phases based on rigid polymeric spheres of divinylbenzene (DVB), Selerity says its Polaratherm can reach its true potential as a separation device.
The enhanced temperature stability of the DVB-based columns means that by incorporating them with the Polaratherm total temperature controller, optimal separation is achieved.
High temperature HPLC has long been proven to greatly optimise separation because of its ability to use temperatures of up to 200C.
However, traditional silica columns have been unable to facilitate this, due to their inability to withstand the elevated temperatures or extreme pH levels which are required for this process.
Stationary phases containing DVB offer superior pH stability, and can be used at levels ranging from pH0-14.
A new application note shows the performance of three stationary phases based on DVB.
All three columns were able to perform at high temperatures.
The columns were exposed to 1000 column volumes of mobile phase at increasing temperatures up to 160C where they began to show a loss of theoretical plates.
This experiment showed that DVB-based columns can be exposed to temperatures as high as 150C without showing any signs of degradation.
Further details about these experiments and their accompanying results can be found in Selerity's new technical note (number 808).
The Polaratherm total temperature controller is an HPLC column compartment which can be integrated with any existing high performance chromatograph at little extra cost.
It is capable of regulating temperatures ranging from subzero to 200C and is completely compatible with most chromatography instrumentation.
