After transduction of the lentivector into mammalian cells, the construct becomes integrated into the cell genome and the reporter gene is expressed if the transcriptional factor is present
New PathNet transcriptional reporter lentivectors from Cambridge BioScience enable researchers to detect the activation of transcription factors in a natural environment (within the cell nucleus).
This novel technology, developed by System Biosciences, uses either an HIV- or FIV-based lentivector for studying signalling pathways.
It can be used in nearly all cell types, including primary and non-dividing mammalian cells.
Following transduction of the lentivector report construct into mammalian cells, the construct becomes stably integrated into the cell genome.
After integration, the reporter gene is expressed if the transcriptional factor is present in this cell type.
Researchers can choose between three different reporters (dscopGFP, luciferase, and beta-galactosidase) under the control of the minimum CMV (mCMV) promoter.
