Kits are designed for gene expression analysis, providing accurate quantification of the expression of target and control genes for up to four targets in a tube or well
Real-time, one-step RT-PCR is taken to the next level by QuantiTect Multiplex RT-PCR kits, which says Qiagen make multiplex analysis absolutely simple.
Up to four RNA targets in a tube or well can be reliably quantified without any tedious optimisation steps.
The kits are designed for gene expression analysis, providing accurate quantification of the expression of target and control genes.
The preoptimised master mix and protocols eliminate the need to adjust reaction and cycling conditions.
The unique components of the master mix promote stable and efficient primer annealing, prevent the formation of nonspecific products and primer-dimers in every PCR cycle, and allow cDNA synthesis from a wide range of RNA template amounts.
Optimal results are achieved even in multiplex reactions to quantify transcripts that differ greatly in abundance.
Target genes and the endogenous control are coamplified with similar efficiencies to ensure highly precise relative quantification.
Low-abundance transcripts can also be quantified, with detection of as few as ten copies possible.
The kits are compatible with all available real-time cyclers and with dual-labelled probes, such as TaqMan probes and QuantiProbes.
Two kits are available for different types of cycler: the QuantiTect Multiplex RT-PCR kit (with Rox dye for fluorescence normalisation) and the QuantiTect Multiplex RT-PCR NR kit (without Rox dye).
