The new Nano-Plotter NP2.1 is said to be perfect for immobilising low volumes of DNA, proteins and cell lysates on any surfaces
Biosensors and micro arrays can be printed with unsurpassed ease and precision using non-contact piezoelectric micropipetting technology with no impact on sensitive surfaces.
Volumes as low as 0.1nl can be dispensed as perfectly spherical droplets.
Applications include:.
Semi-automatic drug screening using adherent cells or tissue slices in the microscope.
Viability tests (eg for cancer cells) and other cell based tests.
Laser manipulation of cells in the flow: DNA transfection and 'optical stretcher' (measurement of deformability of cells for cancer diagnosis and to isolate stem cells).
Cell adhesion tests using prepared surfaces.
Interaction of cells with immobilised proteins, oligosaccharides, lipids and other ligands.
Single molecule detection using fluorescent biomolecules (fluorescence correlation spectroscopy in-the-flow, receptor-ligand binding studies, motor protein kinetics, hybridization kinetics on microarrays etc).
Application of hydrodynamic flow fields (eg alignment of macromolecules, nanotechnology).
Generation of lateral concentration gradients in microchannels.
Measurement of homogeneity of microbeads and sorting.
Activity measurement of electrically active cells on microelectrode arrays in the flow-through.
Rapid prototyping of microsystems (eg for stopped flow or chemical synthesis) by use of PDMS.
'Sample carrier' for the study of non-transparent substrates under the microscope.
Nano-Plotter NP2.1 features:.
No impact on sensitive surfaces through touchless technology.
Unlimited spot replication from each single sample aspiration.
Homogeneous spot topology.
Variation in dosage volume as low as 0.1nl without changing tip.
Flexibility to dispense microlitre quantities through standard disposable tips.
Array layout can be arbitrary and independent of pipette head layout.
Highly effective washing and cleaning system.
New double washbowl rinses the tips with two different wash fluids for more efficient removal of sticky proteins.
Special humidifying and chilling technology (dew point pipetting) prevents evaporation and conserves nanolitre volumes in applications such as miniaturised assays.
Temperature controlled slide deck.
