Magnetic beads separate proteomic samples based on isoelectric point and net charge differences, in preparation for downstream analyses such as mass spectrometry, HPLC or 1D/2D gel electrophoresis
Invitrogen announces the availability of Dynabeads SCX, the newest proteomic sample preparation product from Dynal.
Dynabeads SCX are designed with an optimised surface for binding and fractionation of intricate protein and peptide mixtures.
Using strong cationic exchange (SCX), these magnetic beads precisely separate proteomic samples based on isoelectric point and net charge differences, in preparation for downstream analyses such as mass spectrometry, HPLC or 1D/2D gel electrophoresis.
Dynabeads SCX add further functionality to Dynal's existing magnetic bead chromatography product line, which includes products for cation exchange (Dynabeads WCX), anion exchange (Dynabeads SAX) and hydrophobic capture (Dynabeads RPC 18 and Dynabeads RPC Protein).
Beads with different surface characteristics can be combined to isolate different fractions from the same sample, allowing for step-wise reduction of sample complexity.
"These versatile Dynabeads products can be used to concentrate, prepare and desalt complex protein and peptide mixtures, making them particularly valuable for serum profiling in biomarker and drug discovery," says Beate Rygg Johnsen, business area manager at Dynal.
"The magnetic separation methodology is fast, reproducible, flexible and scalable, and can be automated on a wide variety of liquid handling platforms for both top-down and bottom-up approaches, offering an excellent alternative to chromatography columns, spin columns and pipette tip technologies."
