At the Association of Biomolecular Resource Facilities 2007 annual conference, AnaSpec presented a technical poster that compared the synthesis of a fluorescent peptide using two types of resins
The results of the poster indicated that the use of an unprotected resin was just as effective as using a protected resin for the synthesis of a 5-FAM labelled peptide.
Many Fret (fluorescent resonance energy transfer) peptides, as well as non-Fret peptides, requiring C-terminal fluorescent labels can be accomplished using either solution phase or solid phase synthesis.
In solid phase synthesis, the use of the fluorophore, 5-carboxyfluorescein (5-FAM) generates stable amide bonds.
In order to help facilitate the synthesis of C-terminal fluorescent peptides, AnaSpec prepared and performed comparison studies using two kinds of resins, Fmoc-Lys(5-FAM)-Rink Amide resin (I) and Fmoc-Lys[5-FAM(trt)]-Rink Amide resin (II), the latter contains a phenolic hydroxyl group protected with a trityl group.
The results of these studies showed that resin (I) is adequate for synthesis of C-terminal fluorescent labelled peptide.
