Anaspec has brought out anti-Irak1 (pThr 209) and anti-Irak1 (pThr387), two phosphospecific Irak1 polyclonal antibodies.
These antibodies are specific for the phosphothreonine sequence and not the non-phosphothreonine sequence.
The cross-reactivity between the pThr209 and pThr 387 antibodies is minimal.
Interleukin-1 (IL-1) receptor-associated kinase (Irak) is a serine/threonine-specific kinase.
The mammalian family of Irak molecules contains four members (Irak1, Irak2, Irak3/Irak-M and Irak4).
The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1RI, AcP, MyD88 and Iraks.
Irak undergoes autophosphorylation shortly after IL-1 stimulation and serves as a signalling molecule to mediate IL-1 response.
Irak mediates a signalling cascade leading to NF-KB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF).
Rabbit anti-Irak-1(pThr209) (cat no.
55331) polyclonal antibody was raised against a peptide corresponding to human Irak1 at the phosphorylated threonine 209 (EISRGpTH).
Rabbit anti-Irak-1(pThr387) (cat no.
55333) polyclonal antibody corresponds to human Irak1 at the phosphorylated threonine 387 (VRG-pTLAY).
The non-phosphorylated version of these antibodies is also available.
The Irak1 antibodies have been evaluated for specificity by dot blot.
