Anaspec has introduced a line of secondary antibodies and streptavidin conjugated to the Hilyte Fluor 594: the newest member of the Hilyte Fluor family.
Using optimal fluorophore/protein labelling ratio, these Hilyte Fluor 594-IgG conjugates exhibit high fluorescent signal and uncompromised biological function.
The fluorescence of Hilyte Fluor 594 (Ex/Em = 593/616nm) can be detected with filters designed for Texas Red (Invitrogen).
The labelling performance and stability properties of Hilyte Fluor 594 are better than those of Texas Red, according to the company.
It has a higher extinction coefficient (80,000 M-1cm-1) and a lower correction factor (0.17) compared to Alexa Fluor 594 (Invitrogen).
The fluorescence quantum yield of Hilyte Fluor 594 is 0.9 in aqueous solution.
Biomolecules conjugated to Hilyte Fluor 594 exhibit little spectral overlap with green-fluorescent conjugates and can be efficiently excited by the 568nm line of Ar-Kr laser and by the 594nm line of orange He-Ne laser.
Hilyte Fluor 594-conjugated streptavidin provides high fluorescence intensity and low background, as validated in immunofluorescence staining of mammalian cells.
Hilyte Fluor 594-protein conjugates are suitable for immunofluorescent staining, in-situ hybridisation, flow cytometry and other biological applications.
The Hilyte Fluor 594 is available in a variety of reactive forms, such as acid, amine, C2 maleimide, hydrazide and succinimidyl ester (SE).
