Preanalytix has introduced the Paxgene Blood miRNA Kit, which provides a standardised method for co-purification of RNA and miRNA from human-blood samples.
Currently, many researchers rely on the Paxgene Blood RNA System, consisting of Paxgene Blood RNA Tubes and Paxgene Blood RNA Kits, for collection and stabilisation of human whole blood.
The system inhibits RNA degradation and gene induction in vitro, resulting in preservation of the gene-expression profile that is essential for accurate analysis.
However, researchers cannot easily extract and purify the miRNA from these same samples.
Marie McCluskey, global product manager for pre-analytical systems at Qiagen, said: 'Up to this point, academic, pharma and biobank researchers have been relying primarily on miRNA extraction methods from their blood samples, which do not include a stabilisation step.
'In addition, these methods often lack standardised collection of the sample and as a result lead to a lack of consistency in results.' Paxgene Blood RNA Tubes used in combination with the new Paxgene Blood miRNA Kit comprise the first system available for collecting and stabilising whole human blood and co-purifying total RNA enriched for miRNA.
Paxgene Blood miRNA protocols are available for manual and automated solutions.
For increased efficiency in the lab, this research-use only (RUO) protocol can be automated on Qiagen's QIAcube.
Up to 12 samples can be processed in a single run using the QIAcube variant of the Paxgene Blood miRNA protocol.
The Paxgene Tissue System is available for studies requiring histological and molecular analyses.
The Paxgene Tissue System combines tissue fixation and stabilisation with purification of nucleic acids, including intact miRNA.