Anaspec's Calcein AM (calcein acetoxymethyl ester), a non-fluorescent, cell-permeable compound, converts to the green fluorescent calcein when hydrolysed by intracellular esterases in live cells.
It is widely used for determining cell viability as the fluorescence intensity of calcein is proportional to the amount of live cells.
Calcein is a photostable reagent and is well retained in the cytoplasm of live cells.
It is not influenced by intracellular pH.
Its bright-green fluorescence can be monitored at Ex/Em=494nm/520nm.
Among other reagents, including BCECF, AM and Carboxy-fluorescein diacetate, Calcein AM is a suitable indicator for staining viable cells thanks to its low-cytotoxicity property.
Calcein AM does not affect cell proliferation or viability and is used in cell invasion, adhesion, migration and other cell-based assays.
The company provides Calcein AM at two purity grades - more than 90 per cent or more than 95 per cent - as determined by HPLC.
Anaspec also offers the Sensolyte Calcein Cell Viability Assay Kit: a rapid and sensitive assay to detect viable cells.
This kit provides ample reagents (dye and assay buffer) for 1,000 assays in a 96-well plate format.
