An application note has been released that demonstrates how the Thermo Scientific Nanodrop 3300 has enabled cancer researchers to perform fluorescence measurements on DNA.
The researchers used the Nanodrop 3300 to study DNA methylation events and polyamine-induced DNA G-quadruplex formations by microvolume fluorometry.
The Nanodrop is said to have allowed researchers to perform fluorescence measurements quickly and cost effectively.
The application note reviews methods in the current literature where the Nanodrop 3300 enables scientists to investigate complex DNA structures within the field of cancer research.
It provides an insight into the benefits of using fluorescence spectroscopy for proof-of-concept experimentation of DNA methylation detection assays, as well as DNA G-quadruplex analysis.
Fluorescence spectroscopy methodologies are also aiding the targeting of and delivery to cancer cells in vivo to correct defects, inhibit growth and induce apoptosis.
In addition, the technique provides excellent sensitivity and specificity for researchers working in this field.
The 'cuvette-less' sample retention system employed by the Nanodrop 3300 enables fluorescent measurements using as little as one microlitre sample volume.
The fluorospectrometer is also capable of analysing multiple emission profiles from a single sample.
In addition, the instrument has a small footprint, taking up minimal space in the laboratory.
Thermo Fisher Scientific is offering a free trial program for its instruments, including the Nanodrop 3300, in order to demonstrate the ease of use of its fluorescence technology.
The application note, entitled 'Using the Nanodrop 3300 Fluorospectrometer for Cancer Research Applications', is available for download.
