Bioo Scientific’s NEXTflex 16S V1 – V3 Amplicon-Seq Kit is designed to simplify bacterial metagenomics studies for sequencing on the Illumina MiSeq platform.
The kit allows users to go from sample to sequence in two hours, which Bioo Scientific claims makes it the fastest library prep kit available.
Up to 384 barcodes are available with the kit for flexibility and multiplexing power to rRNA sequencing. Sequencing primers are included in the kit.
16S V1-V3 amplicon processing involves two main steps: an initial PCR amplification using customised PCR primers that target the V1-V3 domains, and a subsequent PCR amplification that integrates relevant flow cell binding domains and unique 12 base pair sample indices.
The number of cleanup steps is limited to ensure maximum recovery of amplicons for downstream sequencing.
The kit is designed to simplify bacterial diversity studies, studies of interactions between host species and bacterial communities, identification of non-culturable bacteria, detection of adventitious agents and enzyme discovery and production.
It is the latest addition to Bioo Scientific’s line of library prep kits for Illumina-compatible metagenomics analysis, which includes the NEXTflex 16S V4 Amplicon-Seq Kits.