Kits provide gene-specific, validated populations of siRNA to knock down key genes in specific biological pathways; reagents for verifying suppression at the RNA level using RT-PCR
SureSilencing siRNA and antibody kits, available from Cambridge BioScience, are designed to verify gene suppression at both the RNA and protein levels using one transfection experiment.
Each kit provides gene-specific, validated populations of siRNA to knock down key genes in specific biological pathways; reagents for verifying suppression at the RNA level using RT-PCR; and protein specific antibodies from Zymed Laboratories for verifying suppression at the protein level by Western blotting.
Most RNA interference products only guarantee silencing of gene expression at the RNA level, but silencing at the protein level is essential to cause effects on the rest of the cell system.
SureSilencing siRNA and antibody kits provide the means to test the level of the targeted protein.
About siRNA: Small interfering RNA is a short RNA duplex between 15-21 nucleotides in length.
Once transfected into cells, siRNA, in conjunction with cellular machinery, targets mRNA molecules that contain an identical sequence and cause degradation in a catalytic manner.
The degraded message is no longer functional in translation and thus expression of the corresponding gene is silenced.
These products are available from Cambridge BioScience in the UK and Ireland only.
