Application note demonstrates how assay and cell analysis system has enabled scientists to quickly and easily study cell migration and screen for drug compounds that affect that process
A new application note describing the actin polymerisation assay
and its use in measuring chemokine-induced chemotaxis is now
available from the Guava Technologies website, in the online
Produced in collaboration with the Leiden
University Medical Centre in the Netherlands, the research
demonstrates how using Guava Express and the Guava PCA cell
analysis system has enabled scientists to quickly and easily
study cell migration and screen for drug compounds that affect
Further the research illustrates how the use of
Guava Express in an actin polymerisation assay can provide an
early measure of chemotaxis (the migration of cells toward or
away from a chemical stimulus), within seconds of exposure to
The researchers conclude that with Guava Express and the
Guava cell analysis system that results can be gained more
quickly and easily than is possible with conventional methods.
Cellular movement in response to chemical signals rpresents a
fundamental property of immune cells because it enables their
mobilisation and deployment to sites of inflammation throughout
This process of chemotaxis can be measured at its
initiation by assaying for actin polymerisation within cells, a
physiological requirement for cell movement.
The actin assay can
be readily adopted to identify drug compounds that enhance or
inhibit chemotaxis or to study pathways involved in chemotaxis.
For observing intracellular proteins or quantifying cell surface
markers, the Guava Express assay is said to provide superior
sensitivity for antigen detection in whole cells.
Even very low
levels of the protein/antigen of interest can be detected,
providing excellent discrimination between cells expressing low
and high levels of protein.
The high sensitivity of the Guava
Express assay is due to precise reagent labelling coupled with
the unique illumination used within the Guava PCA systems.
Express assay uses proprietary Guava Express software, a Guava
PCA system and optimised Express secondary reagents.
Express assay combines sensitivity with easy data analysis to
provide rapid, reliable and objective protein expression results.
The broad dynamic range of the Guava Express assay provides the
precise discrimination necessary for studying cell receptor
up/down-regulation, screening transfectants, evaluating whole
blood lymphocyte populations, and many other applications.
"Chemotaxis is an important area of study for immunologists,
cancer researchers and drug discovery labs," said Angela
Schultz, director of marketing at Guava Technologies.
of the Guava PCA and Guava Express allows researchers to identify
compounds that inhibit chemotaxis in an accurate and reproducible
manner, with much greater ease and speed than can be achieved
using conventional cell migration assays.
"The result is
greater productivity in the lab, and faster decision